The C1V1 = C2V2 calculator allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1. The Dilution refers to making a lower concentration solution from higher concentrations. Solutions usually are stored in a higher concentration, for convenient usage and avoiding contamination. The dilution calculator is based on the following equation:
Concentration(start) x Volume(start) = Concentration(final) x Volume(final)
If the stock concentration is 25 femtomolar, desired concentration is 12 femtomolar and desired volume is 9 microliter, find the required volume?
25 × V1 = 12 × 9
25 × V1 = 108
V1 = 4.32.