Protein Isoelectric Point Calculator

The Henderson-Hasselbalch equation defines the relationship of pH with the acid dissociation constant (pKa), in biological and chemical systems. The equation is useful for estimating the pH of a buffer solution and finding the equilibrium pH in acid-base reactions. The protein isoelectric point calculator calculates the pH at which a particular protein molecule carries no net electrical charge in the statistical mean. Calculation of isoelectric points can be done by entering the values of HCO3 and PaCO2

Calculation of Isoelectric Points

Result :

Example: Calculate the isoelectric point of protein using the values HCO3 is 23 meq/L and PCO2 is 40 mmHg? Henderson Hasselbalch Equation = 6.1 + log ((23)/(0.0301×40)) pH(I) = 9.04984486904252