# Protein Isoelectric Point Calculator

The Henderson-Hasselbalch equation defines the relationship of pH with the acid dissociation constant (pKa), in biological and chemical systems. The equation is useful for estimating the pH of a buffer solution and finding the equilibrium pH in acid-base reactions. The protein isoelectric point calculator calculates the pH at which a particular protein molecule carries no net electrical charge in the statistical mean. Calculation of isoelectric points can be done by entering the values of HCO_{3} and PaCO_{2}

## Calculation of Isoelectric Points

Example: Calculate the isoelectric point of protein using the values HCO

_{3} is 23 meq/L and PCO

_{2} is 40 mmHg?
Henderson Hasselbalch Equation = 6.1 + log ((23)/(0.0301×40))
pH(I) = 9.04984486904252